Membrane-Based Micro-Volume Dialysis Method for Rapid and High-Throughput Protein Crystallization

Protein crystallization techniques are very important in drug development. This paper discusses a membrane-based micro-volume dialysis method suitable for rapid and high-throughput protein crystallization. A droplet of solution was deposited on the membrane surface device. Crystallizing agents could be added to either directly or through membrane. The process easily monitored real time under microscope. Tiny specks, indicative forming crystals, were observed as early 30 s from start experiment, these clearly distinguishable tetragonal lysozyme crystals within 2 min. is particularly carrying out screening optimization experiments, studying kinetics. easy direct manner which crystallizing introduced into device makes this amenable miniaturization automation. Additionally, approach would potentially allow effects molecules nature crystal formation.